Flower-like GO-MoS2 SERS platform for sensitive quantification of cortisol

doi:10.1007/s44275-024-00024-x

Moore and More ›› 2025, Vol. 1 ›› Issue (4): 318-326.DOI: 10.1007/s44275-024-00024-x

• Original Article • 上一篇下一篇

Flower-like GO-MoS₂ SERS platform for sensitive quantification of cortisol

Wenmiao Yu¹, Xuan Xu², Tingting Zheng¹

1. Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, China;
2. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China

收稿日期:2024-10-08 修回日期:2024-12-17 接受日期:2024-12-26 出版日期:2025-11-29 发布日期:2025-03-24
通讯作者: Tingting Zheng,E-mail:ttzheng@chem.ecnu.edu.cn
Wenmiao Yu received her B.S. in chemistry from Donghua University in 2022. In the same year, she attended the School of Chemistry and Molecular Engineering at East China Normal University as a graduate student and conducted research in analytical chemistry under the guidance of Prof. Tingting Zheng. Her research focuses on the analysis of surface-enhanced Raman spectroscopy activity of semiconductor.
Xuan Xu received her B.S. in chemistry from Hubei University in 2021. In the same year, she attended the School of Chemistry and Chemical Engineering at Nanjing University as a graduate student and conducted research in analytical chemistry under the guidance of Prof. Jianrong Zhang. Her research focuses on the application of surfaceenhanced Raman spectroscopy in bioanalysis.
Tingting Zheng received her Ph.D. in analytical chemistry from Nanjing University in 2015. From July 2015, she joined the Department of Chemistry at East China Normal University. She is now a professor in the same department. Her research focuses on the innovation of materials, mechanism analysis, and applications of semiconductor surface-enhanced Raman scattering.
基金资助:
This work was supported by the National Natural Science Foundation of China (22222405).

Flower-like GO-MoS₂ SERS platform for sensitive quantification of cortisol

Wenmiao Yu¹, Xuan Xu², Tingting Zheng¹

1. Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, China;
2. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China

Received:2024-10-08 Revised:2024-12-17 Accepted:2024-12-26 Online:2025-11-29 Published:2025-03-24
Contact: Tingting Zheng,E-mail:ttzheng@chem.ecnu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (22222405).

摘要/Abstract

摘要： Mental stress is a dangerous factor for the health of living beings, which can lead to various diseases. However, currently, there is a lack of diagnostic tools that can quickly and accurately quantify levels of mental stress. Cortisol is an important stress hormone that is widely present in bodily fluids; its concentration can reflect the level of mental stress in organisms. Here, we report a surface-enhanced Raman spectroscopy (SERS) probe based on flower-like graphene oxide-molybdenum disulfide composite material functionalized with cortisol DNA recognition element and tetracyanoquinodimethane of Raman label, with a remarkable enhancement factor value of 7.38 × 10⁵, which exhibits excellent cortisol detection ability in a wide range of concentrations from 1 nM to 1 000 nM, with the limit of detection down to 0.773 nM. The whole detection takes only 20 min. In addition, the SERS probe can selectively detect cortisol in other substances with similar chemical structures, which makes the probe applicable to complex biological systems with good reproducibility and stability. This designed SERS probe has been successfully employed in the detection of mouse serum cortisol, with high accuracy compared with enzyme-linked immunosorbent assay (ELISA) results, demonstrating great potential in actual biological sample detection.

关键词: Surface-enhanced Raman spectroscopy (SERS), Mental stress, Cortisol detection, Charge-transfer effects, Biological sample analysis

Abstract: Mental stress is a dangerous factor for the health of living beings, which can lead to various diseases. However, currently, there is a lack of diagnostic tools that can quickly and accurately quantify levels of mental stress. Cortisol is an important stress hormone that is widely present in bodily fluids; its concentration can reflect the level of mental stress in organisms. Here, we report a surface-enhanced Raman spectroscopy (SERS) probe based on flower-like graphene oxide-molybdenum disulfide composite material functionalized with cortisol DNA recognition element and tetracyanoquinodimethane of Raman label, with a remarkable enhancement factor value of 7.38 × 10⁵, which exhibits excellent cortisol detection ability in a wide range of concentrations from 1 nM to 1 000 nM, with the limit of detection down to 0.773 nM. The whole detection takes only 20 min. In addition, the SERS probe can selectively detect cortisol in other substances with similar chemical structures, which makes the probe applicable to complex biological systems with good reproducibility and stability. This designed SERS probe has been successfully employed in the detection of mouse serum cortisol, with high accuracy compared with enzyme-linked immunosorbent assay (ELISA) results, demonstrating great potential in actual biological sample detection.

Key words: Surface-enhanced Raman spectroscopy (SERS), Mental stress, Cortisol detection, Charge-transfer effects, Biological sample analysis

Wenmiao Yu, Xuan Xu, Tingting Zheng. Flower-like GO-MoS₂ SERS platform for sensitive quantification of cortisol[J]. Moore and More, 2025, 1(4): 318-326.

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Flower-like GO-MoS₂ SERS platform for sensitive quantification of cortisol

Flower-like GO-MoS₂ SERS platform for sensitive quantification of cortisol

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